Single-cell genomic, transcriptomic, and proteomic approaches provide an exciting new avenue for the dissection of the complex set of cell types and states that comprise tissues from essentially organism. However, tissues are not amorphous collections of cells, and the spatial and functional organization of cells within tissues is absolutely critical to the function of all tissues. Thus, to understand tissue function and dysfunction we must know not just the types of cells present within any given tissue but their spatial and functional organization.
Broadly speaking, the mission of our laboratory is to
1) develop novel image-based methods to single-cell 'omics--methods that quantify genome-wide properties of single cells via direct high-resolution fluorescence imaging within intact tissue samples
2) leverage these new technologies to understand how tissue function and dysfunction arise from the behaviors of the individual cells that comprise that system
Below we provide a summary of our basic research directions.