Publications

A list of publications can also be found on pubmed and google scholar.

†Contributed Equally, *Corresponding Author

Featured Papers

A selection of highlights from our work

Highly multiplexed spatial transcriptomics in bacteria

Sarfatis, A., †Wang, Y., Twumasi-Ankrah, N., Moffitt, J.R.

Science, 387 eadr0932 (2025)

bioRxiv (2024)

Charting the cellular biogeography in colitis reveals fibroblast trajectories and coordinated spatial remodeling

Cadinu, P., †Sivanathan, K.N., Misra, A., Xu, R.J., Mangani, D., Yang, E.,Rone, J.M., Tooley, K., Kye, Y., Bod, L., Geistlinger, L., Lee, T., Mertens, R.T., Ono, N., Wang, G., Sanmarco, L., Quintana, F.J., Anderson, A.C., Kuchroo, V.K., *Moffitt, J.R., *Nowarski, R.

Cell, 187 2010 (2024)

bioRxiv (2023)

The emerging landscape of spatial profiling technologies

Moffitt, J.R., Lundberg, E., Heyn, H.

Nature Reviews Genetics 23, 741 (2022)

2025

Protocol optimization improves the performance of multiplexed RNA imaging

Luce, J.J., Reardon-Lochbaum, C.A., Cadinu, P., Xu, R.J., Aceves-Salvador, J., Semizoglou, E., Wong, B., Lopez, I., Cantor, A.B., Renthal, W., Moffitt, J.R.

Scientific Reports, 15 32030 (2025)

bioRxiv (2025)

Inflammatory and anti-inflammatory cytokines bidirectionally modulate amygdala circuits regulating anxiety

Lee, B., Kwon, J.-T., Jeong, Y., Caris, H., Oh, D., Feng, M., Mejia, I.D., Zhang, X., Ishikawa, T., Watson, B.R., Moffitt, J.R., Chung, K., Huh, J.R., Choi, G.B.

Cell, 188 P2190 (2025)

Highly multiplexed spatial transcriptomics in bacteria

Sarfatis, A., †Wang, Y., Twumasi-Ankrah, N., Moffitt, J.R.

Science, 387 eadr0932 (2025)

bioRxiv (2024)

What is the main bottleneck in deriving biological understanding from spatial transcriptomic profiling?

Moffitt, J.R., Li, M., Nie, Q., Kanemaru, K., Teichmann, S.A., Dar, D., Kagohara, L.T., Itzkovitz, S., Vento-Tormo, R., Yanai, I., Fertig, E.J., Theis, F.J.

Cell Systems, 16 101200 (2025)

Spatial transcriptomics of healthy and fibrotic human liver at single-cell resolution

Watson, B., †Paul, B., Rahman, R.U., Amir-Zilberstein, L., Segerstolpe, A.,Epstein, E.T., Murphy, S., Geistlinger, L., Lee, T., Shih, A., Deguine, J., Xavier, R.J., *Moffitt, J.R., *Mullen, A.

Nature Communications, 16 319 (2025)

bioRxiv (2024)

Imaging the intestinal transcriptome with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH)

Cadinu, P., †Yang, E., †Xu, R.J., Watson, B.R., Luce, J., Moffitt, J.R.

Current Protocols, 5 e70111 (2025)

A reference atlas of the human dorsal root ganglion

Bhuiyan, S.A., Nagi, S.S., Sankaranarayanan, I., Semizoglou, E., et al., NIH PRECISION Human Pain Network

bioRxiv doi: https://doi.org/10.1101/2025.11.05.686654 (2025)

An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gut

Xu, R.J., Cadinu, P., Nicol, P.B., Herrmann, U.S., Lee, T., Geistlinger, L., Irizarry, R.A., Moffitt, J.R.

bioRxiv doi: https://doi.org/10.1101/2025.07.21.665958v1 (2025)

Essentialome-wide multigenerational imaging reveals mechanistic origins of cell growth laws

*Eaton, D.S., Sanchez, C., Gutierrez-Lopez, L., Shenker, J.Q., Goulev, Y., Watson, B.R., Henriot, V., Garner, E.C., Justman, Q.A., *Moffitt, J.R., *Paulsson, J.

bioRxiv doi: https://doi.org/10.1101/2025.06.10.658525 (2025)

In situ profiling of plasma cell clonality with image-based single-cell transcriptomics

Yang, E., Aceves-Salvador, J., Castrillon, C., Herrmann, U.S., Akama-Garren, E.H., *Carroll, M.C., *Moffitt, J.R.

bioRxiv doi: https://doi.org/10.1101/2025.05.09.653118 (2025)


2024

Charting the cellular biogeography in colitis reveals fibroblast trajectories and coordinated spatial remodeling

Cadinu, P., †Sivanathan, K.N., Misra, A., Xu, R.J., Mangani, D., Yang, E.,Rone, J.M., Tooley, K., Kye, Y., Bod, L., Geistlinger, L., Lee, T., Mertens, R.T., Ono, N., Wang, G., Sanmarco, L., Quintana, F.J., Anderson, A.C., Kuchroo, V.K., *Moffitt, J.R., *Nowarski, R.

Cell, 187 2010 (2024)

bioRxiv (2023)

Gut complement induced by the microbiota combats pathogens and spares commensals

Wu, M., Zheng, W., Song, X., Bao, B., Wang, Y., Ramanan, D., Yang, D., Liu, R., Macbeth, J.C., Do, E.A., Andrade, W.A., Yang, T., Cho, H.S., Gazzaniga, F.S., Ilves, M., Coronado, D., Thomphson, C., Hang, S., Chiu, I.M, Moffitt, J.R., Hsiao, A., Mekalanos, J.J., Benoist, C., Kasper, D.L.

Cell, 187 897 (2024)

bioRxiv (2023)

Accurate single-molecule spot detection for image-based spatial transcriptomics with weakly supervised deep learning

Laubscher, E., Wang, X., Razin, N., Dougherty, T., Xu, R.J., Ombelets, L., Pao, E., Graf, W. Moffitt, J.R., Yue, Y., Van Valen, D.

Cell Systems, 15 P475 (2024)

bioRxiv (2023)

Identifying spatially variable genes by projecting to morphologically relevant curves

Nicol, P.B., Ma, R., Xu, R.J., Moffitt, J.R., Irizarry, R.A.

bioRxiv doi: https://doi.org/10.1101/2024.11.21.624653 (2024)


2023

Understanding prefrontal cortex functions by decoding its molecular, cellular and circuit organization

Bhattacherjee, A., Zhang, C., Watson,B., Djekidel, M.N., *Moffitt, J.R., *Zhang, Y.

Nature Neuroscience, 26 1880 (2023)

bioRxiv (2022)

Spatial enrichment of the type 1 interferon signature in the brain of a neuropsychiatric lupus murine model

Aw, E., Zhang, Y., Yalcin, E., Herrmann, U., Lin, S.L., Langston, K., Castrillion, C., Ma, M., Moffitt, J.R., Carroll, M.C.

Brain, Behavior, and Immunity, 114 511 (2023)

Spatial organization of the mouse retina at single cell resolution

Choi, J., Li, J., Ferdous, S., Liang, Q., Moffitt, J.R., Chen, R.

Nature Communications 14 4929 (2023)

bioRxiv (2022)

Spatial and temporal profiling of the complement system uncovered novel functions of the alternative complement pathway in brain development

Zhang, Y., Watson, B., Rattan, A., Lee, T., Chawla, S., Geistlinger, G., Guan, Y., Ma, M., Caldarone, B.J., Song, W., *Moffitt, J.R., *Carroll, M.C.

bioRxiv doi: https://doi.org/10.1101/2023.11.22.568325 (2023)


2022

The emerging landscape of spatial profiling technologies

Moffitt, J.R., Lundberg, E., Heyn, H.

Nature Reviews Genetics 23, 741 (2022)


2021

Cell segmentation in imaging-based spatial transcriptomics

Petukhov, V., Xu, R.J., Soldatov, R.A., Cadinu, P., Khodosevich, K., Moffitt, J.R., Kharchenko, P.V.

Nature Biotechnology doi: https://doi.org/10.1038/s41587-021-01044-w (2021)


2020

MAFG-driven astrocytes promote CNS inflammation

Wheeler, M.A., Clark, I.C., Tjon, E.C., Li, Z., Zandee, S.E.J., Couturier, C.P., Watson, B.R., Scalisi, G., Alkwai, S., Rothhammer, V., Rotem, A., Heyman, J.A., Thaploo, S., Sanmarco, L.M., Ragoussis, J., Weitz, D.A., Petrecca, K., Moffitt, J.R., Becher, B., Antel, J.P., Prat, A., Quintana, F.J.

Nature 578 593 (2020)

National Cancer Institute think-tank meeting report on proteomic cartography and biomarkers at the single-cell level: interrogation of premalignant lesions

Kagan, J., Moritz, R., Mazurchuk, R., Lee, J.H., Kharchenko, P., Rozenblatt-Rosen, O., Ruppin, E., Edfors, F., Ginty, F., Goltsev, Y., Wells, J., LaCava, J., Rieseterer, J., Germain, R., Shi, T., Chee, M., Budnik, B., Yates, J., Chait, B., Moffitt, J.R., Smith, R., Srivastava, S.

Journal of Proteome Research 19 1900 (2020)

Spatial organization of the transcriptome in individual neurons

Wang, G., Ang C.-E., Fan, J., Wang, A., Moffitt, J.R., Zhuang, X.

bioRxiv doi: https://doi.org/10.1101/2020.12.07.414060 (2020)


2019

Multiplexed detection of RNA using MERFISH and branched DNA amplification

Xia, C., Babcock, H., *Moffitt, J.R., *Zhuang, X.

Scientific Reports 9 7721 (2019)

bioRxiv (2018)

Copyright 2025

Jeffrey Moffitt